Human IL-32 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB30401
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 373802
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑32 alpha
Phe3-Lys131
Accession # NP_001012651
Phe3-Lys131
Accession # NP_001012651
Specificity
Detects the alpha isoform of IL-32 in direct ELISAs. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) IL-32 beta and rhIL‑32 gamma is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human IL-32 alpha Antibody
Detection of Human IL‑32 alpha by Western Blot.
Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) treated (+) with 200 ng/mL Ionomycin and 10 ng/mL PMA for 72 hours. PVDF Membrane was probed with 2 µg/mL of Human IL-32a Monoclonal Antibody (Catalog # MAB30401) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-32a at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑32 alpha in Human PBMCs.
IL-32a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-32a Monoclonal Antibody (Catalog # MAB30401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human IL-32 alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Western Blot
2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC) treated with Ionomycin and PMA
Sample: Human peripheral blood mononuclear cells (PBMC) treated with Ionomycin and PMA
Reviewed Applications
Read 1 review rated 2 using MAB30401 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-32 alpha
IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.
Long Name
Interleukin 32 alpha
Alternate Names
IL32 alpha
Entrez Gene IDs
9235 (Human)
Gene Symbol
IL32
UniProt
Additional IL-32 alpha Products
Product Documents for Human IL-32 alpha Antibody
Product Specific Notices for Human IL-32 alpha Antibody
For research use only
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