Recombinant Human IL-34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-34 Monoclonal Antibody (Catalog # MAB5265). The ND₅₀ is typically 0.01-0.05 µg/mL.

Applications for Human IL-34 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human IL-34 transfected NS0 cells fixed with paraforaldehyde and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References

Lin, H. et al. (2008) Science 320:807.

Long Name

Interleukin 34

Alternate Names

C16orf77, IL34

Entrez Gene IDs

146433 (Human); 76527 (Mouse)

Gene Symbol

IL34

UniProt

Q6ZMJ4 (Human)

Additional IL-34 Products

Product Documents for Human IL-34 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-34 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-34 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5265

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-34 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human IL-34 Antibody

Cell Proliferation Induced by IL-34 and Neutralization by Human IL-34 Antibody.