Recombinant Human IL-34 Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 5265-IL
Key Product Details
Product Specifications
Source
Asn21-Pro242, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.75-3.75 ng/mL.
Scientific Data Images for Recombinant Human IL-34 Protein
Bioactivity of Human IL-34
Recombinant human IL-34 (5265-IL) induces proliferation in human peripheral blood monocytes. The ED50 for this effect is 0.75-3.75 ng/mL.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 5265-IL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 5265-IL/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N‑linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit‑macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).
References
- Lin, H. et al. (2008) Science 320:807.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-34 Products
Product Documents for Recombinant Human IL-34 Protein
Product Specific Notices for Recombinant Human IL-34 Protein
For research use only