Human IL-36 alpha/IL-1F6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1078
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑36 alpha/IL‑1F6
Met1-Phe158
Accession # Q9UHA7
Met1-Phe158
Accession # Q9UHA7
Specificity
Detects human IL‑36 alpha/IL‑1F6 in direct ELISAs and Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human (rh) rhIL-36 beta, rhIL-36 gamma, rhIL-1 alpha, rhIL-1 beta and rhIL-18 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IL-36 alpha/IL-1F6 Antibody
Detection of Human IL‑36 alpha/IL‑1F6 by Western Blot.
Western blot shows lysates of human tonsil tissue, HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.IL‑36 alpha/IL‑1F6 in Human Tonsil.
IL-36a/IL-1F6 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human IL-36 alpha/IL-1F6 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Western Blot
1 µg/mL
Sample: Human tonsil tissue and HEK293 human embryonic kidney cell line transfected with human IL‑36 alpha/IL‑1F6
Sample: Human tonsil tissue and HEK293 human embryonic kidney cell line transfected with human IL‑36 alpha/IL‑1F6
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-36 alpha/IL-1F6
References
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 alpha/Interleukin 1 Family 6
Alternate Names
FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A
Gene Symbol
IL36A
UniProt
Additional IL-36 alpha/IL-1F6 Products
Product Documents for Human IL-36 alpha/IL-1F6 Antibody
Product Specific Notices for Human IL-36 alpha/IL-1F6 Antibody
For research use only
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