Human IL-38/IL-1F10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2427
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Bioassay, Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑38/IL‑1F10
Cys2-Trp152
Accession # Q8WWZ1
Cys2-Trp152
Accession # Q8WWZ1
Specificity
Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1 F9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IL-38/IL-1F10 Antibody
Human IL‑38/IL‑1F10 ELISA Standard Curve.
Recombinant Human IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-38/IL-1F10 Monoclonal Antibody(Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-38/IL-1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human IL-38/IL-1F10 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑38/IL‑1F10 Monoclonal Antibody(Catalog # MAB24271).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-38/IL-1F10 DuoSet ELISA Kit (Catalog # DY9110-05) for convenient development of a sandwich ELISA.
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL‑38/IL‑1F10
Sample: Recombinant Human IL‑38/IL‑1F10
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-38/IL-1F10
Long Name
Interleukin 1 Family Member 10
Alternate Names
FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38
Gene Symbol
IL1F10
UniProt
Additional IL-38/IL-1F10 Products
Product Documents for Human IL-38/IL-1F10 Antibody
Product Specific Notices for Human IL-38/IL-1F10 Antibody
For research use only
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