Human IL-3R alpha/CD123 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB301N

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 32703

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-3 R alpha/CD123
Lys20-Arg305, predicted
Accession # P26951

Specificity

Detects human IL-3 R alpha/CD123 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of IL-3 Ra/CD123 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of IL-3 R alpha/CD123 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (FAB4869P) and either (A) Mouse Anti-Human IL-3 Ra/CD123 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB301N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (IC002N). View our protocol for Staining Membrane-associated Proteins.

Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry

THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB301N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Human peripheral blood lymphocytes; THP-1 human acute monocytic leukemia cell line 
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References

  1. Moore, M.A.S. et al. (1991) Blood 72:944.
  2. Warren, D.J. et al. (1988) J. Immunol. 140:94.
  3. Plant M. et al. (1989) Nature 339:150.
  4. Budel, L.M. et al. (1990) Blood 75:1439.
  5. Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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