Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (FAB4869P) and either (A) Mouse Anti-Human IL-3 Ra/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry

THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 APC-conjugated Monoclonal Antibody (Catalog # FAB301A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-3R alpha/CD123 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes; THP-1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References

Moore, M.A.S. et al. (1991) Blood 72:944.
Warren, D.J. et al. (1988) J. Immunol. 140:94.
Plant M. et al. (1989) Nature 339:150.
Budel, L.M. et al. (1990) Blood 75:1439.
Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents for Human IL-3R alpha/CD123 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-3R alpha/CD123 APC-conjugated Antibody

For research use only

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Spectra Viewer
Flow Cytometry

Human IL-3R alpha/CD123 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB301A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-3R alpha/CD123 APC-conjugated Antibody

Detection of IL‑3 R alpha/CD123 in Human Blood Lymphocytes by Flow Cytometry.