Human IL-6 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC206P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 1936
Product Specifications
Immunogen
E. coli-derived recombinant human IL-6
Val30-Met212
Accession # P05231
Val30-Met212
Accession # P05231
Specificity
Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IL-6 PE-conjugated Antibody
Detection of IL-6 in PBMCs by Flow Cytometry.
PBMCs stimulated with 100 ng/ml LPS for 24 hours and Brefeldin A for 5 hours (A) or Naïve PBMCs (B) were stained with Mouse Anti-Human CD14 Alexa Fluor® 750-conjugated Monoclonal Antibody (Catalog # FAB3832S) and Mouse Anti-Human IL-6 PE-conjugated Monoclonal Antibody (Catalog # IC206P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-6 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: PBMCs stimulated with 100 ng/ml LPS for 24 hours and Brefeldin A for 5 hours or Naïve PBMCs
Sample: PBMCs stimulated with 100 ng/ml LPS for 24 hours and Brefeldin A for 5 hours or Naïve PBMCs
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-6
Long Name
Interleukin 6
Alternate Names
BSF-2, BSF2, IFNB2, IL6, MGI-2A
Entrez Gene IDs
Gene Symbol
IL6
UniProt
Additional IL-6 Products
Product Documents for Human IL-6 PE-conjugated Antibody
Product Specific Notices for Human IL-6 PE-conjugated Antibody
For research use only
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