Human IL-6R alpha (Research Grade Tocilizumab Biosimilar) Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB11482V
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu20
Product Specifications
Immunogen
E. coli-derived recombinant human IL-6R alpha
Arg387-Arg468
Accession # P08887
Arg387-Arg468
Accession # P08887
Specificity
Detects human IL-6R alpha in direct ELISAs.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Applications
Application
Recommended Usage
Flow Cytometry
Titration recommended for optimal concentration with starting
range of 0.1-1 µg/1 million cells. Sample used for this experiment was HEK293 cells transfected with hIL-6Ra and eGFP.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium
Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-6R alpha
The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex (1‑4). Within the extracellular domain, human IL‑6 R alpha shares 52% aa sequence identity with
mouse and rat IL‑6 R alpha.
A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL‑6/IL‑6 R alpha complex to membrane-bound gp130 (6, 7).
References
- Yamasaki et al. (1988) Science 241:825.
- Baumann et al. (1990) J. Biol. Chem. 265:19853.
- Hibi et al. (1990) Cell 63:1149.
- Schooltink et al. (1991) Eur. J. Biochem. 277:659.
- Novick et al. (1989) J. Exp. Med. 170:1409.
- Yasukawa et al. (1990) J. Biochem. 108:673.
- Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
UniProt
Additional IL-6R alpha Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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