Detects human IL-9 R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL‑13 R alpha1, rhIL-13 R alpha2, or recombinant mouse IL-9 R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-9R Antibody

Detection of IL-9 R in Human Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Mouse Anti-Human IL-9 R Subunit Monoclonal Antibody (Catalog # MAB290, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Cell Proliferation Induced by IL-9 and Neutralization by Human IL-9 R Antibody.

Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-9 R Subunit Mono-clonal Antibody (Catalog # MAB290). The ND₅₀ is typically 2-4 µg/mL.

Applications for Human IL-9R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human whole blood monocytes

Neutralization

Measured by its ability to neutralize IL-9-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 2-4 µg/mL in the presence of 2 ng/mL Recombinant Human IL-9.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB290 in the following applications:

Flow Cytometry (1 Review)
Functional Assay (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9R

Interleukin 9 Receptor (IL-9 R) belongs to the hematopoietin receptor superfamily and is the binding subunit of the heterodimeric IL-9 receptor complex. The other subunit is the common gamma chain shared with the receptors for IL-2, IL-4, IL-7, and IL-15. IL-9 R is expressed by T cells, neutrophils, mast cells, and macrophages.

Long Name

Interleukin 9 Receptor

Alternate Names

CD129, IL-9 R, IL9R

Entrez Gene IDs

3581 (Human); 16199 (Mouse)

Gene Symbol

IL9R

Additional IL-9R Products

Product Documents for Human IL-9R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-9R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IL-9R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB290

Key Product Details

Species Reactivity

Validated:

Cited:

Applications