Human Importin alpha3/KPNA4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8204
Key Product Details
Species Reactivity
Validated:
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 843124
Product Summary for Human Importin alpha3/KPNA4 Antibody
Immunogen
E. coli-derived recombinant human Importin alpha3/KPNA4
Met1-Asn104
Accession # O00629
Met1-Asn104
Accession # O00629
Specificity
Detects human Importin alpha3/KPNA4 in ELISA and Western blot. In direct ELISA, no cross-reactivity to human KPNA3 was detected.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Importin alpha3/KPNA4 Antibody
Detection of Human Importin alpha3/KPNA4 by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin a3/KPNA4 Monoclonal Antibody (Catalog # MAB8204) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin a3/KPNA4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Importin alpha3/KPNA4 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Importin alpha3/KPNA4 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Importin alpha3/KPNA4 Monoclonal Antibody (Catalog # MAB8204). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human Importin alpha3/KPNA4 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Importin alpha 3/KPNA4
Long Name
Karyopherin alpha 4
Alternate Names
KPNA4, QIP12, SRP3
Gene Symbol
KPNA4
UniProt
Additional Importin alpha 3/KPNA4 Products
Product Documents for Human Importin alpha3/KPNA4 Antibody
Product Specific Notices for Human Importin alpha3/KPNA4 Antibody
For research use only
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