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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # IA3

Product Specifications

Immunogen

Human milk epithelial cell line

Specificity

Detects human Integrin alpha3/CD49c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha3/CD49c Biotinylated Antibody

Integrin a3/CD49c antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC).

Integrin alpha3/CD49c in HT1080 Human Cell Line.

Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Biotinylated Monoclonal Antibody (Catalog # BAM1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Integrin  alpha3/CD49c Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 3/CD49c

The alpha3 subunit, also known as CD49c and VLA-3 alpha subunit, forms a non-covalent heterodimer with the Integrin beta1 subunit (CD29). Integrin alpha3 beta1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin.

Alternate Names

CD49c, ITGA3

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Gene Symbol

ITGA3

Additional Integrin alpha 3/CD49c Products

Product Documents for Human Integrin  alpha3/CD49c Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha3/CD49c Biotinylated Antibody

For research use only

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