Human Integrin alpha4/CD49d Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA37
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Adhesion Blockade
Cited:
Blocking, Flow Cytometry, Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2B4
Product Specifications
Immunogen
U937 human histiocytic lymphoma cell line
Specificity
Detects human Integrin alpha4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication 2:87).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human Integrin alpha4/CD49d Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized recombinant human VCAM-1 (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 5 μg/mL of the antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 4/CD49d
Integrin alpha4, also known as CD49d, is a transmembrane protein that forms heterodimers with beta1 or beta7 integrins. These complexes contribute to the trafficking of many hematopoietic cell types. Integrins alpha4 beta1 (also known as VLA-4) and alpha4 beta7 (LPAM-1) are receptors for fibronectin, VCAM and MAdCAM-1.
Alternate Names
CD49d, ITGA4, VLA-4
Gene Symbol
ITGA4
Additional Integrin alpha 4/CD49d Products
Product Documents for Human Integrin alpha4/CD49d Antibody
Product Specific Notices for Human Integrin alpha4/CD49d Antibody
For research use only
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