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Human Integrin  alpha4/CD49d Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1354

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 7.2R

Product Specifications

Immunogen

DX-3 human melanoma cell line

Specificity

Detects human Integrin alpha4/CD49d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha4/CD49d Biotinylated Antibody

Integrin  alpha4/CD49d in MOLT-4 Human Cell Line.

Integrin  alpha4/CD49d was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human Integrin  alpha4/CD49d Biotinylated Monoclonal Antibody (Catalog # BAM1354) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin  alpha4/CD49d Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta1 (CD29) or beta7. Integrins alpha4 beta1 and alpha4 beta7 are receptors for fibronectin and VCAM. Integrin alpha4 beta1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Alternate Names

CD49d, ITGA4, VLA-4

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Gene Symbol

ITGA4

Additional Integrin alpha 4/CD49d Products

Product Documents for Human Integrin  alpha4/CD49d Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha4/CD49d Biotinylated Antibody

For research use only

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