Human Integrin alpha5/CD49e Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1864
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Adhesion Blockade, CyTOF-ready, Flow Cytometry, Simple Western, Western Blot
Cited:
Co-Immunoprecipitation, ELISA Capture, Immunocytochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha5/CD49e
Phe42-Tyr995
Accession # P08648
Phe42-Tyr995
Accession # P08648
Specificity
Detects human Integrin alpha5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) Integrin alpha5 and less than 5% cross-reactivity with recombinant human Integrin alphaM, rmIntegrin alpha2, rmIntegrin alpha4, and rmIntegrin alphaE is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Integrin alpha5/CD49e Antibody
Detection of Human Integrin a5/CD49e by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Integrin alpha5/CD49e by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (U87 MG) (NBP2-49844), Exosome Standards (PC-3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin alpha5/CD49e at approximately 165 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin alpha5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Human Integrin alpha5/CD49e by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha5/CD49e at approximately 165 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin alpha5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Integrin alpha5/CD49e Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Simple Western
20 µg/mL
Sample: Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Sample: Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Reviewed Applications
Read 2 reviews rated 4.5 using AF1864 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 5/CD49e
Alternate Names
CD49e, ITGA5, VLA-5 alpha
Gene Symbol
ITGA5
UniProt
Additional Integrin alpha 5/CD49e Products
Product Documents for Human Integrin alpha5/CD49e Antibody
Product Specific Notices for Human Integrin alpha5/CD49e Antibody
For research use only
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