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Human Integrin  alpha5/CD49e Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1864

R&D Systems, part of Bio-Techne
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BAF1864

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin alpha5/CD49e
Phe42-Tyr995
Accession # P08648

Specificity

Detects human Integrin alpha5/CD49e in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin alpha5 is observed and less than 2% cross-reactivity with recombinant human Integrin alphaM, rmIntegrin alpha2, rmIntegrin alpha4, and rmIntegrin  alphaE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Integrin  alpha5/CD49e Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin  alpha5/CD49e

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The alpha5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta1 subunit (CD29). Integrin alpha5 beta1 is a receptor for fibronectin and neural adhesion molecule L1.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin  alpha5/CD49e Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha5/CD49e Biotinylated Antibody

For research use only

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