K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin a5/CD49e APC-conjugated Monoclonal Antibody (Catalog # FAB1864A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha5/CD49e APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 5/CD49e

The Integrin alpha5 subunit, also known as CD49e, associates with the Integrin beta1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References

Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Te Velde, A. et al. (1988) J. Immunol. 140:1548.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P08648

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin alpha5/CD49e APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Integrin alpha5/CD49e APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Integrin alpha5/CD49e APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1864A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Integrin alpha 5/CD49e Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha5/CD49e APC-conjugated Antibody

Detection of Integrin alpha5/CD49e in K562 Human Cell Line by Flow Cytometry.