K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin alpha 5/CD49e Monoclonal Antibody (Catalog # MAB1864, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Integrin alpha5/CD49e in IMR-90 Human Cell Line.

Integrin alpha5/CD49e was detected in immersion fixed IMR-90 human lung fibroblast cell line using Mouse Anti-Human Integrin alpha5/CD49e Monoclonal Antibody (Catalog # MAB1864) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin alpha5/CD49e Antibody

Application

Recommended Usage

Adhesion Activation

The adhesion of K562 human chronic myelogenous leukemia cells (5 x 10⁴ cells/well) to immobilized Bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by pre-treatment of cells with 500 ng/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed IMR‑90 human lung fibroblast cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The Integrin alpha5 subunit, also called CD49e, associates with the Integrin beta1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References

Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Te Velde, A. et al. (1988) J. Immunol. 140:1548.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P08648

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin alpha5/CD49e Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Integrin alpha5/CD49e Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha5/CD49e Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1864

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha 5/CD49e Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human Integrin alpha5/CD49e Antibody

Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.