Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin aL/CD11a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3595F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alphaL/CD11a Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha L/CD11a

Integrin subunit alphaL/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM-1 through -4. Human Integrin alphaL contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alphaL ECD share 74% aa identity. CD11a has two splice variants, one which contains a 53 aa insert after Gln954, and a second which shows a deletion of aa 110-192.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Entrez Gene IDs

3683 (Human); 16408 (Mouse); 308995 (Rat)

Gene Symbol

ITGAL

UniProt

CAA68747

Additional Integrin alpha L/CD11a Products

Product Documents for Human Integrin alphaL/CD11a Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Integrin alphaL/CD11a Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Integrin alphaL/CD11a Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3595F

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Integrin alpha L/CD11a Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alphaL/CD11a Fluorescein-conjugated Antibody

Detection of Integrin alphaL/CD11a in Human Blood Lymphocytes by Flow Cytometry.