Human Integrin  alphaV/CD51 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1219

R&D Systems, part of Bio-Techne
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BAF1219
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AF1219
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Integrin alpha V/CD51 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alphaV/CD51
Phe31-Val992
Accession # NP_002201

Specificity

Detects human Integrin alphaV/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin  alphaV is observed, 5% cross‑reactivity with recombinant human (rh) Integrin alpha2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha5 and rmIntegrin alphaE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin  alphaV/CD51 Biotinylated Antibody

Integrin aV/CD51 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Integrin alphaV/CD51 in Human Mesenchymal Stem Cells.

Integrin aV/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin aV/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin  alphaV/CD51 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunocytochemistry

5-15 µg/mL
Sample:  Immersion fixed human mesenchymal stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin  alphaV/CD51
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alphaV, also known as CD51 and vitronectin receptor subunit  alpha, is a 140 - 150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alphaV, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta-propellar domain. Furin cleavage of the alphaV ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alphaV chain. alphaV-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alphaV shares 92% aa sequence identity with mouse Integrin  alphaV ECD.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Gene Symbol

ITGAV

UniProt

NP_002201

Additional Integrin alpha V/CD51 Products

Product Documents for Human Integrin  alphaV/CD51 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Integrin  alphaV/CD51 Biotinylated Antibody

For research use only

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