Human Integrin beta4/CD104 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4060A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 422325
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta4/CD104
Asn28-Ser710
Accession # P16144
Asn28-Ser710
Accession # P16144
Specificity
Detects human Integrin beta4/CD104 in Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta1, beta2, beta3, beta5, beta6, beta8, or recombinant mouse Integrin beta4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Integrin beta4/CD104 APC-conjugated Antibody
Detection of Integrin beta4/CD104 in A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Integrin beta4/CD104 APC-conjugated Monoclonal Antibody (Catalog # FAB4060A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin beta4/CD104 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Integrin beta 4/CD104
Integrin beta4 (also GP150 and CD104) is a 150‑200 kDa member of the integrin beta family of proteins. It forms noncovalent heterodimers with integrin alpha6 and participates in the formation of epithelial hemidesmosomes by binding Plexin 1A. Integrin alpha6 beta4 also binds Laminin. Human integrin beta4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa 28‑710) and a large 1089 aa cytoplasmic region. One ECD alternate splice form is reported. One shows an 84 aa substitution for the 84 aa between aa 621‑704. A second shows a 144 aa substitution for the first 704 amino acids. Human beta4 ECD shares 88% aa sequence identity with mouse beta4 ECD.
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
UniProt
Additional Integrin beta 4/CD104 Products
Product Documents for Human Integrin beta4/CD104 APC-conjugated Antibody
Product Specific Notices for Human Integrin beta4/CD104 APC-conjugated Antibody
For research use only
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