Human Integrin beta5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3824
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta5
Gly24-Asn719
Accession # P18084
Gly24-Asn719
Accession # P18084
Specificity
Detects human Integrin beta5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin beta1, rhIntegrin beta3, rhIntegrin beta4, rhIntegrin beta6 and rmIntegrin beta7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta5 Antibody
Integrin beta5 in MG‑63 Human Cell Line.
Integrin beta5 was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Integrin beta5 by Simple WesternTM.
Simple Western lane view shows lysates of MG-63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta5 at approximately 129 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Integrin beta5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Integrin beta5 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Integrin beta5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin beta5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Integrin beta5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: MG-63 human osteosarcoma cell line
Sample: MG-63 human osteosarcoma cell line
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line
Sample: Immersion fixed MG-63 human osteosarcoma cell line
Simple Western
50 µg/mL
Sample: MG‑63 human osteosarcoma cell line
Sample: MG‑63 human osteosarcoma cell line
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Reviewed Applications
Read 1 review rated 5 using AF3824 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 5
Integrin beta5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alphaV. Integrin alphaV beta5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta5, respectively.
Alternate Names
ITGB5
Gene Symbol
ITGB5
UniProt
Additional Integrin beta 5 Products
Product Documents for Human Integrin beta5 Antibody
Product Specific Notices for Human Integrin beta5 Antibody
For research use only
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