Human Integrin beta7 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4669
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta7 long isoform
Glu20-His723
Accession # P26010
Glu20-His723
Accession # P26010
Specificity
Detects human Integrin beta7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin beta7 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin beta2, rhIntegrin beta5, and rhIntegrin beta6 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Integrin beta7 Antibody
Detection of Integrin beta7 in Human Lymphocytes by Flow Cytometry.
Human blood derived lymphocytes were stained with Human Integrin beta7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4669, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).Applications for Human Integrin beta7 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human lymphocytes
Sample: Human lymphocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin beta7
Sample: Recombinant Human Integrin beta7
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 7
Human Integrin beta7 (also Integrin betaP and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20-723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa 501-648. Integrin beta7 forms a nondisulfide linked heterodimer with alpha4 and alphaE integrins. alpha4 beta7 binds fibronectin, VCAM-1, and MADCAM1, while alphaE beta7 binds E-cadherin. The ECD of human beta7 is 87% aa identical to the ECD of mouse beta7.
Alternate Names
ITGB7
Gene Symbol
ITGB7
UniProt
Additional Integrin beta 7 Products
Product Documents for Human Integrin beta7 Antibody
Product Specific Notices for Human Integrin beta7 Antibody
For research use only
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