Human IRAK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4048
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IRAK1
Met1-Ser693
Accession # AAH54000
Met1-Ser693
Accession # AAH54000
Specificity
Detects human IRAK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK2 and rhIRAK4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IRAK1 Antibody
Detection of Human IRAK1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (10 ng/lane) were included. A specific band for IRAK1 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IRAK1 by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK1 at approximately 83 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human IRAK1 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line
Sample: K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRAK1
IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4.
Long Name
Interleukin-1 Receptor-Associated Kinase 1
Alternate Names
Il1rak, Pelle, Plpk
Gene Symbol
IRAK1
UniProt
Additional IRAK1 Products
Product Documents for Human IRAK1 Antibody
Product Specific Notices for Human IRAK1 Antibody
For research use only
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