Human IRAK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6690
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 600848
Product Specifications
Immunogen
E. coli-derived recombinant human IRAK2
Ala2-Asn590
Accession # O43187
Ala2-Asn590
Accession # O43187
Specificity
Detects human IRAK2 in direct ELISAs and Western blots.
In direct ELISAs, no cross-reactivity
with recombinant human (rh) IRAK1, 3, or 4 is observed.
In Western blots, less than 25%
cross-reactivity with rhIRAK1, 3, and 4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human IRAK2 Antibody
Detection of Human IRAK2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRAK2 Monoclonal Antibody (Catalog # MAB6690) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRAK2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IRAK2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRAK2
Long Name
Interleukin-1 Receptor-Associated Kinase 2
Alternate Names
interleukin-1 receptor associated kinase-2, interleukin-1 receptor-associated kinase 2, interleukin-1 receptor-associated kinase-like 2, IRAK-2, MGC150550
Gene Symbol
IRAK2
UniProt
Additional IRAK2 Products
Product Documents for Human IRAK2 Antibody
Product Specific Notices for Human IRAK2 Antibody
For research use only
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