Human IRF8 Antibody

Detection of Human IRF8 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 25 µg of nuclear extracts (Nuc) and whole cell lysate (WCL). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRF8 in Human Tonsil.

IRF8 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IRF8 by Simple Western^TM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for IRF8 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.