Human IRS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6347

R&D Systems, part of Bio-Techne
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MAB6347
MAB6347-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 676415

View all IRS2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRS2
Pro1035-Ile1136
Accession # Q9Y4H2

Specificity

Detects human IRS2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IRS1 or recombinant mouse IRS3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human IRS2 Antibody

Detection of Human IRS2 antibody by Western Blot.

Detection of Human IRS2 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IRS2 Monoclonal Antibody (Catalog # MAB6347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRS2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IRS2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaN3 with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS2

IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules.  It is the primary IRS protein found in hematopoietic cells.  Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding.  This event allows for its subsequent association with PI-3 kinase.  Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa.  It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075).  IRS2 is phosphorylated on multiple Ser and Tyr residues.  There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338.  Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. 

Long Name

Insulin Receptor Substrate 2

Alternate Names

insulin receptor substrate 2, IRS-2

Entrez Gene IDs

8660 (Human); 384783 (Mouse); 29376 (Rat)

Gene Symbol

IRS2

UniProt

Q9Y4H2 (Human)

Additional IRS2 Products

Product Documents for Human IRS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRS2 Antibody

For research use only

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