Human IRS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6347

R&D Systems, part of Bio-Techne
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AF6347
AF6347-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all IRS2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRS2
Pro1035-Ile1136
Accession # Q9Y4H2

Specificity

Detects human IRS2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IRS2 Antibody

Detection of Human IRS2 antibody by Western Blot.

Detection of Human IRS2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human IRS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6347) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRS2 at approximately 170-190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human IRS2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRS2

IRS2 (insulin receptor substrate 2) is a 180-190 kDa substrate intermediate that lies between various cytokine receptors and downstream signaling molecules. It is the primary IRS protein found in hematopoietic cells. Depending upon the receptor, IRS2 undergoes phosphorylation, either via Tyk2 following type I IFN binding, or via JAK1 and 3 following IL-4, -7 and -15 binding. This event allows for its subsequent association with PI-3 kinase. Human IRS2 is 1338 amino acids (aa) in length with a predicted MW of 138 kDa. It contains one PH (pleckstrin homology) domain (aa 16-144), followed by a PTB (phosphotyrosine-binding)domain (aa 194-298) and seven YxxM (Tyr/x/x/Met) motifs (aa 540-1075). IRS2 is phosphorylated on multiple Ser and Tyr residues. There are two potential splice variants. One shows a 16 aa substitution for the C-terminal Glu, while another shows an 11 aa substitution for aa 1314-1338. Over aa 1035-1136, human IRS2 shares 86% aa identity with mouse IRS2. 

Long Name

Insulin Receptor Substrate 2

Alternate Names

insulin receptor substrate 2, IRS-2

Entrez Gene IDs

8660 (Human); 384783 (Mouse); 29376 (Rat)

Gene Symbol

IRS2

UniProt

Q9Y4H2 (Human)

Additional IRS2 Products

Product Documents for Human IRS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRS2 Antibody

For research use only

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