Human ISG15/UCRP APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8044A

R&D Systems, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Western Blot

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 851701

View all ISG15/UCRP Antibodies »

Product Specifications

Immunogen

Full-length recombinant human ISG15/UCRP

Accession # P05161

Specificity

Detects human ISG15/UCRP in Elisa and Western Blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human ISG15/UCRP APC-conjugated Antibody

Detection of ISG15/UCRP antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line untreated (light orange filled histogram) or treated with 500 U/mL Recombinant Human IFN-beta 1a (Mammalian) (Catalog # 11415-1) for 16 hours (dark orange filled histogram) was stained with Rat Anti-Human ISG15/UCRP APC-conjugated Monoclonal Antibody (Catalog # IC8044A) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human ISG15/UCRP APC-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line treated with Recombinant Human IFN‑ beta 1a (Mammalian) (Catalog # 11415-1) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ISG15/UCRP

ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Entrez Gene IDs

9636 (Human); 100038882 (Mouse); 298693 (Rat)

Gene Symbol

ISG15

UniProt

P05161 (Human)

Additional ISG15/UCRP Products

Product Documents for Human ISG15/UCRP APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ISG15/UCRP APC-conjugated Antibody

For research use only

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