Human ITM2A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4876
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ITM2A
Pro80-Glu263
Accession # O43736
Pro80-Glu263
Accession # O43736
Specificity
Detects human ITM2A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ITM2B is observed, and less than 1% cross-reactivity with rhITM2C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ITM2A Antibody
Detection of Human ITM2A by Western Blot.
Western Blot shows lysates of A-673 human Ewing's sarcoma cell line. PVDF membrane was probed with 2 µg/ml of Sheep Anti-Human ITM2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4876) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2A at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human ITM2A Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A-673 human Ewing's sarcoma cell line
Sample: A-673 human Ewing's sarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ITM2A
ITM2A (Integral membrane protein 2A; also protein E25) is a 43-45 kDa member of the ITM2 family of proteins. It is expressed by chondrocytes involved in endochondrial ossification, skeletal muscle, adipose tissue-derived stem cells, and CD3-activated CD4+ and CD8+ T cells. ITM2A is found embedded in both plasma membrane and Golgi complex. Human ITM2A is a 263 amino acid (aa) type II transmembrane glycoprotein. The ECD (aa 75-263) contains one BRICHOS domain (aa 133‑227). Proteolytic cleavage by furin is typical for BRICHOS domain-containing proteins. ITM2A may exist as a dimer. There are two potential splice variants, one that shows a deletion of aa 55-81, and a second that shows a deletion of aa 38-81. Full-length human ITM2A shares 95% aa identity with mouse ITM2A.
Long Name
Integral Membrane Protein 2A
Alternate Names
BRICD2A, E25A
Gene Symbol
ITM2A
UniProt
Additional ITM2A Products
Product Documents for Human ITM2A Antibody
Product Specific Notices for Human ITM2A Antibody
For research use only
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