Human Jak3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4699
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 452506
Product Specifications
Immunogen
E. coli-derived recombinant human Jak3
Gly46-Thr209
Accession # P52333
Gly46-Thr209
Accession # P52333
Specificity
Detects human Jak3 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Jak3 Antibody
Detection of Human Jak3 by Western Blot.
Western blot shows lysates of TF-1 human erythroleukemic cell line. PVDF membrane was probed with 2 µg/mL of Human Jak3 Monoclonal Antibody (Catalog # MAB4699) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jak3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human Jak3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: TF-1 human erythroleukemic cell line
Sample: TF-1 human erythroleukemic cell line
Reviewed Applications
Read 2 reviews rated 3.5 using MAB4699 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jak3
Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID).
Alternate Names
JAKL, LJAK
Gene Symbol
JAK3
UniProt
Additional Jak3 Products
Product Documents for Human Jak3 Antibody
Product Specific Notices for Human Jak3 Antibody
For research use only
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