Human KA2/GRIK5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11334

R&D Systems, part of Bio-Techne
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MAB11334-100
MAB11334-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1060109

View all KA2/GRIK5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence
Accession # Q16478

Specificity

Detects human KA2/GRIK5 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human KA2/GRIK5 Antibody

Detection of KA2/GRIK5 in Human Hippocampus.

KA2/GRIK5 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human KA2/GRIK5 Monoclonal Antibody (Catalog # MAB11334) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human KA2/GRIK5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KA2/GRIK5

GRIK5 (Glutamate receptor, ionotropic kainate 5) is a receptor for glutamate and is one of the 5 types of kainate receptor subunits.  L-Glutamate acts as an excitatory neurotransmitter at many synapses in the CNS. The GRIK5 protein forms functional heteromic kainate-preferring ionic channels with the subunits encoded by related gene family members.  Kainate receptors have both presynaptic and postsynaptic actions and a limited distribution in the brain. Kainate receptors have been shown to have connections with several neurological diseases and conditions.  There is linkage to schizophrenia, depression, autism, Huntington's, bipolar disorder, and epilepsy among others.  KA2 is relatively abundant in all areas of the brain, most notably in dentate gyrus, pyramidal neurons of CA3, cerebellar granule cells, as well as being present in superficial and deep laminae of the neocortex.

References

  1. Entrez Gene: GRIK5 glutamate receptor, ionotropic, kainate 5.
  2. Szpirer C, Molne M, Antonacci R, Jenkins NA, Finelli P, Szpirer J, Riviere M, Gilbert DJ, Copeland NG, et al. "The Genese Encoding the Glutamate Receptor Subunits Ka1 and KA2 (GRIK4 and GRIK5) are located on separate chromosomes in Human, Mouse and Rat". Proc Natl Acad Sci U.S.A. 1995 Jan; 91(25):11849.
  3. Contractor A, Mulle C, Swanson GT. "Kainate Receptors Coming of Age: Milestones of Two Decades of Research". Trends in Neurosciences. 2011 Mar; 34(3):154.
  4. Matute C. "Therapeutic Potential of Kainate Receptors". CNS Neuroscience & Therapeutics. 2011 Dec; 17(6):661.

Long Name

Glutamate Receptor KA2

Alternate Names

EAA2, GluK5, GRIK2, GRIK5

Entrez Gene IDs

2901 (Human); 14809 (Mouse); 24407 (Rat)

Gene Symbol

GRIK5

UniProt

Q16478 (Human)

Additional KA2/GRIK5 Products

Product Documents for Human KA2/GRIK5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human KA2/GRIK5 Antibody

For research use only

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