Human Kallikrein 5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1108
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Bioassay, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5
Ile67-Ser293
Accession # Q9Y337
Ile67-Ser293
Accession # Q9Y337
Specificity
Detects human Kallikrein 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse KLK5 is observed and less than 10% cross-reactivity with recombinant human KLK8 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Kallikrein 5 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 5 (Catalog # 1108-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 5 (Catalog # 1108-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Kallikrein 5 (Catalog # 1108-SE)
Sample: Recombinant Human Kallikrein 5 (Catalog # 1108-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 5
Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).
References
- Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
- Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
- Kim, H. et al. (2001) Br. J. Cancer 84:643.
Alternate Names
KLK-L2, KLK5, SCTE
Gene Symbol
KLK5
UniProt
Additional Kallikrein 5 Products
Product Documents for Human Kallikrein 5 Antibody
Product Specific Notices for Human Kallikrein 5 Antibody
For research use only
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