Human Kallikrein 5 Propeptide Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11082
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 325302
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 5
Val23-Arg66
Accession # NP_036559
Val23-Arg66
Accession # NP_036559
Specificity
Detects human Kallikrein 5 Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 5 (mature), rhKallikrein 1, -3, -4, -6, -7, -8, -9, -10, -11, -12, -13, -14, -15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin, or rhuPA-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Kallikrein 5 Propeptide Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Kallikrein 5 (Catalog # 1108-SE)
Sample: Recombinant Human Kallikrein 5 (Catalog # 1108-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 5
Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).
References
- Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
- Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
- Kim, H. et al. (2001) Br. J. Cancer 84:643.
Alternate Names
KLK-L2, KLK5, SCTE
Gene Symbol
KLK5
UniProt
Additional Kallikrein 5 Products
Product Documents for Human Kallikrein 5 Propeptide Antibody
Product Specific Notices for Human Kallikrein 5 Propeptide Antibody
For research use only
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