Human Kallikrein 7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2624
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
ELISA Development, Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 333931
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 7
Glu23-His252
Accession # P49862
Glu23-His252
Accession # P49862
Specificity
Detects human Kallikrein 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Kallikrein 1, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, or 15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Kallikrein 7 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 7 (Catalog # 2624-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 7 (Catalog # 2624-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Kallikrein 7 (Catalog # 2624-SE) under non-reducing conditions only
Sample: Recombinant Human Kallikrein 7 (Catalog # 2624-SE) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB2624 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kallikrein 7
Human tissue Kallikrein 7 (hK7), also known as stratum corneum chymotryptic enzyme (SCCE), is a member of the human tissue kallikrein family. Full-length Kallikrein 7 consists of 253 amino acids (aa), with a signal peptide (aa 1-22), short pro peptide (aa 23-29) and mature chain (aa 30-252) (1). Predominantly expressed in the skin, a major physiological function of Kallikrein 7 is to regulate the desquamation process through proteolysis of the intercellular adhesive structures between corneocytes (2). Thus, it is related to some inflammatory skin diseases, such as psoriasis and chronic itchy dermatitis (3, 4). Studies have shown that one potential physiological activator for Kallikrein 7 is Kallikrein 5, another member of the human tissue Kallikrein family. Along with Kallikrein 14, these three Kallikreins form a proteolytic cascade in the stratum corneum (5).
References
- Hansson, L. et al. (1994) J. Bio. Chem. 269:19420.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.
- Ekholm, E. and T. Egelrud (1999) Arch. Dermatol. Res. 291:195.
- Hansson, L. et al. (2002) J. Invest. Dermatol. 118:444.
- Brattsand, M. et al. (2004) J. Invest. Dermatol. 124:198.
Alternate Names
KLK7
Gene Symbol
KLK7
UniProt
Additional Kallikrein 7 Products
Product Documents for Human Kallikrein 7 Antibody
Product Specific Notices for Human Kallikrein 7 Antibody
For research use only
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