Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
R&D Systems, part of Bio-Techne | Catalog # ES002
Substrate for MMP-3, MMP-10, Trypsin, HGF Activator, and Coagulation Factor X
Key Product Details
Conjugate
7-Methoxycoumarin
Applications
Binding Activity, Bioactivity
Product Specifications
Source
Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2
Mca: (7-Methoxycoumarin-4-yl)acetyl, Nval: Norvaline, Dnp: 2, 4-Dinitrophenyl.
Purity
>95%, by HPLC.
Predicted Molecular Mass
1676 Da
Activity
The peptide substrate contains a highly fluorescent
7-methoxycoumarin group that is efficiently quenched by resonance energy
transfer to the 2,4-dinitrophenyl group. It can be used to measure the
activities of peptidases that are capable of cleaving an amide bond between the
fluorescent group and the quencher group, causing an increase in fluorescence.
It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).
It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).
It is also an excellent substrate for Trypsin, HGF activator and factor Xa.
Reviewed Applications
Read 3 reviews rated 5 using ES002 in the following applications:
Formulation, Preparation and Storage
ES002
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light. |
Background: Protease Substrates
Additional Protease Substrates Products
Product Documents for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
Product Specific Notices for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
For research use only
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