Human Katanin p60 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7100
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 717705
Product Specifications
Immunogen
E. coli-derived recombinant human Katanin p60
Asp308-Tyr378
Accession # O75449
Asp308-Tyr378
Accession # O75449
Specificity
Detects human Katanin p60 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Katanin p60 subunit A-like 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Katanin p60 Antibody
Katanin p60 in Human Brain.
Katanin p60 was detected in immersion fixed paraffin-embedded sections of human brain (globus pallidus) using Mouse Anti-Human Katanin p60 Monoclonal Antibody (Catalog # MAB7100) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Katanin p60 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (globus pallidus)
Sample: Immersion fixed paraffin-embedded sections of human brain (globus pallidus)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Katanin p60
KATNA1 (Katanin [Japanese word for sword] p60 ATPase-containing subunit A1; also Katanin p60) is a 55-60 kDa member of the VPS4 subfamily, AAA ATPase family of molecules. It is ubiquitously expressed and is recognized to sever microtubules, allowing for their reorganization during cell division and migration. KATNA1 is most effective on nonacetylated, non-tau binding microtubules. In general, KATNA1 activity is regulated by binding to KATNB1/katanin p80, which potentiates KATNA1 action. However, the relationship is complex, and governed by the local p60:p80 ratio. Human KATNA1 is 491 amino acids (aa) in length. It contains a p80 interaction segment (aa 1-29), followed by a microtubule interaction region (aa 30-185), an ATPase domain (aa 239-381), and an oligomerization region (aa 455-489). KATNA1 is phosphorylated on Ser170. There is one isoform variant that shows a deletion of aa 168-243 coupled to a four aa substitution for aa 384-491. Over aa 308-378, human KATNA1 shares 97% aa identity with mouse KATNA1.
Long Name
Katanin p60 (ATPase containing) Subunit A 1
Alternate Names
KATNA1
Gene Symbol
KATNA1
UniProt
Additional Katanin p60 Products
Product Documents for Human Katanin p60 Antibody
Product Specific Notices for Human Katanin p60 Antibody
For research use only
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