Human Kell Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1914
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kell
aa 68-732
aa 68-732
Specificity
Detects human Kell in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kell is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Kell Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood
Sample: Human whole blood
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kell, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Kell, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Kell
Sample: Recombinant Human Kell
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kell
Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).
References
- Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
- Turner, A.J. et al. (2001) BioEssays. 23:261.
- Lee, S. et al. (1999) Blood 94:1440.
- Lee, S. et al. (2001) J. Biol. Chem. 276:27281.
Long Name
Zinc protease blood group antigen
Alternate Names
CD238, KEL
Gene Symbol
KEL
Additional Kell Products
Product Documents for Human Kell Antibody
Product Specific Notices for Human Kell Antibody
For research use only
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