Human Ketohexokinase Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8177

R&D Systems, part of Bio-Techne
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MAB8177-100
MAB8177-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1020613

View all Ketohexokinase Antibodies »

Product Specifications

Immunogen

E. coli-derived human Ketohexokinase
Met1-Val298
Accession # P50053

Specificity

Detects human Ketohexokinase in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Ketohexokinase Antibody

Detection of Human Ketohexokinase antibody by Western Blot.

Detection of Human Ketohexokinase by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ketohexokinase Monoclonal Antibody (Catalog # MAB8177) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ketohexokinase at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ketohexokinase antibody in Human Liver by Immunohistochemistry (IHC-P).

Ketohexokinase in Human Liver.

Ketohexokinase was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Ketohexokinase Monoclonal Antibody (Catalog # MAB8177) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Ketohexokinase Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ketohexokinase

KHK1(Ketohexokinase) catalyzes conversion of fructose to fructose-1-phosphate (1). It is the first enzyme that catabolizes dietary fructose. Mutation of this protein is the molecular basis for essential fructosuria, a clinically benign condition characterized by the incomplete metabolism of fructose in the liver, leading to its excretion in urine (2, 3). Essential fructosuria does not have any clinical manifestations and no treatment is required. However, deficiency of aldolase B, the second enzyme involved in the metabolism of fructose results in the accumulation of fructose-1-phosphate in the blood, which causes fructosemia or hereditary fructose intolerance (4). High level of fructose-1-phosphate inhibits the production of glucose and results in diminished regeneration of adenosine triphosphate. Patients with fuctosemia have symptoms of elevated uric acid, growth abnormalities, and coma if untreated. Therefore, inhibition of KHK1 may lead to a cure for fructosemia. High level of expression of KHK1 is found in liver, kidney, gut, spleen and pancreas. Low levels of expression of KHK1 is found in heart, muscle, brain, and eye (3). The enzymatic activity of recombinant human KHK1 is measured using a phosphatase-coupled method (5).

References

  1. Trinh, C.H. et al. (2009) Acta. Crystallogr. D Biol Crystallogr. 65:201.
  2. Zhang, X. et al. (2011) Bioorg. Med. Chem. Lett. 21:4762.
  3. Bonthron, D.T. et al. (1994) Hum. Mol. Genet. 3:1627.
  4. Kaiser, U.B. and Hegele, R. A. (1991). Am. J. Med. Sci. 302: 364.
  5. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

Fructokinase, KHK

Entrez Gene IDs

3795 (Human); 16548 (Mouse); 25659 (Rat)

Gene Symbol

KHK

UniProt

P50053

Additional Ketohexokinase Products

Product Documents for Human Ketohexokinase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Ketohexokinase Antibody

For research use only

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