Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human KIR2DL3/CD158b2 Antibody

Detection of KIR2DL3/CD158b2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL3/CD158b2 Monoclonal Antibody (Catalog # MAB2014) or (B) Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P).View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DL3/CD158b2 Antibody

Application

Recommended Usage

CyTOF-reported

Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human whole blood CD56⁺ natural killer cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB2014 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DL3/CD158b2

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158b2, NKAT2

Entrez Gene IDs

3804 (Human)

Gene Symbol

KIR2DL3

UniProt

P43628

Additional KIR2DL3/CD158b2 Products

Product Documents for Human KIR2DL3/CD158b2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KIR2DL3/CD158b2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KIR2DL3/CD158b2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2014

Key Product Details

Species Reactivity

Validated:

Cited:

Applications