Human KLF4 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3640A
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human KLF4
Ala2-Phe470
Accession # AAH29923
Ala2-Phe470
Accession # AAH29923
Specificity
Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) KLF4 and less than 1% cross-reactivity with recombinant human (rh) KLF5, rhKLF6, and rmKLF15.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human KLF4 APC-conjugated Antibody
Detection of KLF4 in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Goat Anti-Human KLF4 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3640A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human KLF4 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KLF4
Long Name
Kruppel-Like Factor 4
Alternate Names
EZF
Entrez Gene IDs
9314 (Human)
Gene Symbol
KLF4
UniProt
Additional KLF4 Products
Product Documents for Human KLF4 APC-conjugated Antibody
Product Specific Notices for Human KLF4 APC-conjugated Antibody
For research use only
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