Human Klotho beta Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB58891R
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Rabbit IgG Clone # 1025C
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived recombinant human Klotho beta with a C-terminal 10 His tag.
Phe53-Leu997
Accession # Q86Z14
Phe53-Leu997
Accession # Q86Z14
Specificity
Detects human Klotho beta in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
Detection of Klotho beta in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Klotho beta Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB58891R, filled histogram) or isotype control antibody (Catalog # IC1051R, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Klotho beta
Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1, 2). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (3). The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-5). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2-4, 6). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes (1, 7). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (8). The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.
References
- Mian, I. S. (1998) Blood Cells Mol. Dis. 24:83.
- Kurosu, H. and M. Kuro-o (2009) Mol. Cell. Endocrinol. 299:72.
- Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
- Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
- Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
- Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
- Chang, Q. et al. (2005) Science 310:490.
- Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Alternate Names
KLB
Gene Symbol
KLB
UniProt
Additional Klotho beta Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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