KOR was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with KOR (positive staining) and HEK293 human embryonic kidney cell line (wild type, negative staining) using Mouse Anti-Human KOR Monoclonal Antibody (Catalog # MAB3895) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human KOR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human KOR transfected HEK293 cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with KOR

Reviewed Applications

Read 1 review rated 5 using MAB3895 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KOR

KOR is a 45 kDa 7TM opioid receptor that is primarily expressed in the central nervous system and peripheral visceral pain sensory nerves. Following ligation by dynorphin peptides, KOR signaling induces analgesia, dysphoria, diuresis, and increased feeding desire. KOR also exerts neuroprotective and anti-inflammatory effects. Human KOR shares 94% amino acid sequence identity with mouse and rat KOR.

Long Name

Kappa Opioid Receptor

Alternate Names

OPRK1, Oprk2, R21

Entrez Gene IDs

4986 (Human); 18387 (Mouse); 29335 (Rat)

Gene Symbol

OPRK1

UniProt

P41145

Additional KOR Products

Product Documents for Human KOR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human KOR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human KOR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3895

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human KOR Antibody

KOR in HEK293 Human Cell line Transfected with KOR.