Human Kynureninase Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4887

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
MAB4887
MAB4887-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 589731

View all Kynureninase Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynureninase
Met1-Asn465
Accession # Q16719

Specificity

Detects human Kynureninase in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Kynureninase is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Kynureninase Antibody

Detection of Human Kynureninase antibody by Western Blot.

Detection of Human Kynureninase by Western Blot.

Western blot shows lysates of human liver tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kynureninase Monoclonal Antibody (Catalog # MAB4887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Kynureninase at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Kynureninase Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human liver tissue and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3‑hydroxyanthranilic acid and alanine. KYNU and other “kynurenine pathway” enzymes degrade dietary tryptophan in the liver and are involved in the de novo biosynthesis of NAD+. KYNU and other pathway proteins in immune system cells, such as macrophages and microglia, catalyze inflammatory quinolinic acid (QA) production, which may cause neuronal damage in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease.  Human KYNU shares 83% and 85% amino acid identity with mouse and human KYNU, respectively.

Alternate Names

KYNU, L-kynurenine hydrolase

Entrez Gene IDs

8942 (Human); 70789 (Mouse); 116682 (Rat)

Gene Symbol

KYNU

UniProt

Q16719

Additional Kynureninase Products

Product Documents for Human Kynureninase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Kynureninase Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.