Human Kynureninase Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4887R

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB4887R-100
MAB4887R-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 589731R

View all Kynureninase Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynureninase
Met1-Asn465
Accession # Q16719

Specificity

Detects human Kynureninase in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Kynureninase Antibody

Detection of Human Kynureninase antibody by Western Blot.

Detection of Human Kynureninase by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kynureninase Monoclonal Antibody (Catalog # MAB4887R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Kynureninase Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3‑hydroxyanthranilic acid and alanine. KYNU and other “kynurenine pathway” enzymes degrade dietary tryptophan in the liver and are involved in the de novo biosynthesis of NAD+. KYNU and other pathway proteins in immune system cells, such as macrophages and microglia, catalyze inflammatory quinolinic acid (QA) production, which may cause neuronal damage in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease.  Human KYNU shares 83% and 85% amino acid identity with mouse and human KYNU, respectively.

Alternate Names

KYNU, L-kynurenine hydrolase

Entrez Gene IDs

8942 (Human); 70789 (Mouse); 116682 (Rat)

Gene Symbol

KYNU

UniProt

Q16719

Additional Kynureninase Products

Product Documents for Human Kynureninase Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kynureninase Antibody

For research use only

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