Human Laminin alpha3/Laminin-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2144
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Adhesion Blockade, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P3H9-2
Product Specifications
Immunogen
Human keratinocytes
Specificity
Detects human Laminin alpha3/Laminin‑5 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Laminin alpha3/Laminin-5 Antibody
Laminin alpha3/Laminin-5 in MCF 10A Human Cell Line.
Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Laminin alpha3/Laminin‑5 in Human Skin.
Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Laminin alpha3/Laminin-5 Antibody
Application
Recommended Usage
Adhesion Blockade
Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF 10A human breast epithelial cell line
Sample: Immersion fixed MCF 10A human breast epithelial cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Immunoprecipitation
Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654.
Western Blot
Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems.
Reviewed Applications
Read 3 reviews rated 4.7 using MAB2144 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 3/Laminin-5
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha3, beta3, and gamma2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).
References
- Colognato, H. et al. (2000) Dev. Dyn. 218:213.
- Stahl, S. et al. (1997) J. Cell Sci. 110:55.
- Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
Alternate Names
Epiligrin, LAMA3, Laminin-5, Laminin5
Entrez Gene IDs
3909 (Human)
Gene Symbol
LAMA3
Additional Laminin alpha 3/Laminin-5 Products
Product Documents for Human Laminin alpha3/Laminin-5 Antibody
Product Specific Notices for Human Laminin alpha3/Laminin-5 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...