Human LAP TGF-beta 1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM2462

R&D Systems, part of Bio-Techne
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BAM2462
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

Bioassay, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # 27240

View all LAP (TGF-beta 1) Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137

Specificity

Recognizes human LAP (TGF-beta 1) and human latent TGF-beta 1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human LAP TGF-beta 1 Biotinylated Antibody

Application
Recommended Usage

Human LAP TGF-beta 1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human LAP (TGF-beta 1) Antibody (Catalog # MAB2461)
  • Standard: Recombinant Human LAP (TGF-beta 1) Protein (Catalog # 246-LP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using BAM2462 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70 - 80% sequence homology) from mammalian, avain or Xenopus systems that are now designated TGF-beta 1, TGF-beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4 and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with LAP. These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

UniProt

P01137 (Human)

Additional LAP (TGF-beta 1) Products

Product Documents for Human LAP TGF-beta 1 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAP TGF-beta 1 Biotinylated Antibody

For research use only

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