Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human Latrophilin 2/LPHN2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Latrophilin 2/LPHN2 Monoclonal Antibody (Catalog # MAB105881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Latrophilin 2/LPHN2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of LPHN2 in HEK293 Human Cell Line transfected with Human LPHN2 by Flow Cytometry.

HEK293 Human Cell Line transfected with Human LPHN2 was stained with Mouse Anti-Human LPHN2 Monoclonal Antibody (Catalog # MAB105881, filled histogram) or isotype control antibody (MAB10041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Latrophilin 2/LPHN2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Human LPHN2

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human Latrophilin 2/LPHN2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latrophilin 2/LPHN2

Latrophilin 2 (LPHN2) is a G-protein coupled receptor which functions in both cell adhesion and signal transduction. Latrophilin represents a phylogenetically conserved family of receptors which has an unusual structure consisting of two fragments that are produced by proteolytic cleavage of the parental molecule and behave independently in the plasma membrane. LPHN2 is a ubiquitous receptor for alpha-latroxin which is a protein toxin in black widow spider venom that is toxic to vertebrates.

Long Name

Latrophilin 2

Alternate Names

ADGRL2, CIRL2, LEC1, LPHH1, LPHN2

Entrez Gene IDs

23266 (Human)

Gene Symbol

ADGRL2

UniProt

O95490

Additional Latrophilin 2/LPHN2 Products

Product Documents for Human Latrophilin 2/LPHN2 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human Latrophilin 2/LPHN2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Latrophilin 2/LPHN2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB105881

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Latrophilin 2/LPHN2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Latrophilin 2/LPHN2 Antibody

Detection of Human Latrophilin 2/LPHN2 by Western Blot.