Human LBP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF870
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LBP
Ala26-Val481
Accession # AAD21962
Ala26-Val481
Accession # AAD21962
Specificity
Detects human LBP in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse LBP is observed and less than 1% cross-reactivity with recombinant human BPI is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LBP Antibody
Detection of Human LBP by Western Blot
Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LBP by Western Blot
Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human LBP Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human LBP (Catalog # 870-LP)
Sample: Recombinant Human LBP (Catalog # 870-LP)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LBP
Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.
References
- Hailman, E. et al. (1994) J. Exp. Med. 179:269.
- Jack, R.S. et al. (1997) Nature 389:742.
- Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.
Long Name
Lipopolysaccharide-binding Protein
Alternate Names
lipopolysaccharide binding protein, lipopolysaccharide-binding protein, LPS-binding protein, MGC22233
Gene Symbol
LBP
UniProt
Additional LBP Products
Product Documents for Human LBP Antibody
Product Specific Notices for Human LBP Antibody
For research use only
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