Human LC3B Antibody

Name: Human LC3B Antibody
Brand: R&D Systems
SKU: MAB11606

R&D Systems, part of Bio-Techne | Catalog # MAB11606

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Human
Immunogen:	Synthetic Peptide Accession # Q9GZQ8
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 1082854
Specificity:	Detects a synthetic peptide specific for human LC3B around amino acid 10 in Direct ELISA.

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Technical Data
Citations / Reviews

Applications for Human LC3B Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	A172 human glioblastoma cell line untreated (-) or treated (+) with 50 μM Chloroquine for 18 hours

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human LC3B Antibody

	Western Blot Detection of Human LC3B by Western Blot. Western Blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 50µM Chloroquine for 18 hours. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human LC3B Monoclonal Antibody (Catalog # MAB11606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LC3B at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LC3B

Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy.

Item	Value
References	Shpilka, T. et al. (2011) Genome Biol. 12:226. He, H. et al. (2003) J. Biol. Chem. 278:29278. Kuznetsov, S.A. & V.I. Gelfand (1987) FEBS Let. 212:145. Weidberg, H. et al. (2011) Ann Rev. Biochem. 80:125. Weidberg, H. et al. (2010) EMBO J. 29:1792.
Long Name	Microtubule-associated Protein 1 Light Chain 3 beta
Entrez Gene IDs	81631 (Human); 67443 (Mouse); 64862 (Rat)
Alternate Names	ATG8F, Apg8b, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, LC3II, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1LC3B, MAP1LC3B-a, lc3-i, ii, lc3b autophagy marker, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B

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Human LC3B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11606

Key Product Details

Applications for Human LC3B Antibody

Scientific Data Examples for Human LC3B Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: LC3B

Customer Reviews for Human LC3B Antibody

Product Documents for Human LC3B Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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